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Pluripotent stem cell

Real pictures of pluripotent stem cells delivered by gather.site: embryonic and induced lines, their colonies and the structures they form, each with its paper.

14 pictures and 10 papers.

Galleries within: Blastoid (1), iPSC (1)

Gallery

Representative brightfield images of day 4 structures, showing the effects of N2B27, 2% DMSO, and PDA83 conditions on cyst formation in SC9N. Scale bar, 400 µm. (human (SC9N stem cells); Fig. 1, panel b (brightfield day 4 structures, cyst formation; rows mid-section of figure).)

Alsolami, Chandrasekaran, Jin et al 2026, Cell Research · CC BY 4.0

Morphology of Urine-Derived iPSC Colonies. (A) Urine-derived stem cells (USCs) at passage 3 (p3) before Sendai virus (SeV) transfection, serving as a control. Scale bar: 100 µm. (B) Early u-iPSC aggregates on Day 5 after SeV transfection. Scale bar: 500 µm. (C) u-iPSC colony at passage 9 (p9) Day 3. Scale bar: 100 µm. (human (urine-derived induced pluripotent stem cells); Fig. 3, full figure (panels A-C; urine-derived iPSC colony morphology progression).)

Beiner, Zhu, Bosholm et al 2026, FEBS Open Bio · CC BY 4.0

(A) Phase contrast of u-iPSC at p6. All images taken at magnification 5x. Scale bar: 100 µm. (human (urine-derived iPSC at passage 6); Fig. 4, A (phase contrast only; cols 3-351 of figure).)

Beiner, Zhu, Bosholm et al 2026, FEBS Open Bio · CC BY 4.0

(B) Representative immunofluorescence staining for pluripotency markers OCT3/4, NANOG, and SSEA-4 in newly generated PVOD-derived iPSC lines (34L2, 34L3, 34L4). (human (PVOD-derived iPSC lines); Figure 1, panel B (IF pluripotency markers OCT3/4, NANOG, SSEA-4; PVOD-derived iPSC lines 34L2, 34L3, 34L4).)

Lian, Daniels, Wanner et al 2026, Frontiers in Cell and Developmental Biology · CC BY 4.0

Expression of the pluripotent marker in AF-iPSC. (A) Bright field and (B) AP staining of iPS-AF2143. (C) The expression of pluripotent marker OCT-4, SOX-2, and NANOG in iPS-AF2143 by RT-qPCR. (D) The expression of pluripotent marker TRA-1-60, OCT-4, SOX-2 in IPS-AF by immunofluorescence staining. DAPI stained the nucleus (blue). (E) Flow cytometric analysis of pluripotency markers. Histograms showing the expression of TRA-1-60, TRA-1-81, and SSEA-4 in the iPS-AF2143 line compared to the H1. (Figure 3.)

Chen, Jiang, Xue et al 2026, Stem Cells International · CC BY 4.0

Generation of chicken iPSCs using the T7 transcription factor cocktail. (A) Morphological progression of chicken iPSC colony formation by all seven transcription factors (T7: Oct4, Klf4, Sox2, Sox3, Myc, Nanog, Lin28) at different stages: Passage 0 (P0) day 5 (initial colony appearance), Passage 0 (P0) day 7 (colonies ready for picking), and Passage 1 (P1) day 1 (reseeded colonies). Scale bars, 50 um. (B) Quantification of colony numbers generated by different transcription factor combinations. (C) Representative phase-contrast images of colonies generated under selected conditions: all T7 factors (All), no Lin28B, no Sox2, and no Sox3. Colonies lacking Sox3 displayed neuronal-like morphology, indicating differentiation rather than pluripotent reprogramming. Scale bars, 50 um. (D) Representative morphology of a chicken iPSC line at passage 40 (P40). Scale bar, 50 um. (Figure 2.)

Tong, Chen, Anicete et al 2026, Cells · CC BY 4.0

PD/DFA maintains self-renewal and pluripotency in mESCs. (A) AP staining of mESCs cultured for 7 days in 10erum-containing medium supplemented with 5 uM DFA or 1000 U/mL LIF; medium without LIF served as the vehicle control. Scale bar: 100 um. (B) AP staining of mESCs cultured for 7 days in 10erum-containing medium with a concentration gradient of DFA (3, 5, 10, and 15 uM); medium without LIF served as the vehicle control. Scale bar: 100 um. (C) AP staining of mESCs treated for two passages with small-molecule combinations (CHIR, PD, 2i, DFA, CHIR/DFA, PD/DFA, and 2i/DFA). Scale bar: 100 um. (D) Immunofluorescence staining of the self-renewal marker Oct4 in mESCs under the conditions described in (C). Scale bar: 100 um. (E) Western blot analysis of pluripotency factors (Oct4, Klf4, Sox2, and Nanog) in mESCs cultured with PD or PD/DFA. beta-tubulin was used as the loading control. (F) Immunofluorescence staining of lineage-specific markers (Tuj1, Gata4, and Myosin) in cells derived from embryoid bodies generated from PD/DFA-treated mESCs. Scale bar: 100 um. (G) Karyotype of mESCs maintained in PDF for 50 passages, showing a normal diploid chromosome number (2n = 40). (H) Immunofluorescence staining of gamma-H2AX in mESCs cultured under PD or PDF conditions to assess DNA double-strand breaks and genomic stress. Scale bar: 50 um. (I) Mouse blastocysts cultured in G1-Plus medium with a concentration gradient of DFA (5, 10, and 15 uM); G1-Plus medium alone served as the vehicle control. Red arrows indicate developmentally delayed blastocysts. Scale bar: 50 um. (FIGURE 1.)

Zhang, Ji, Dong et al 2026, Advanced Science · CC BY 4.0

Generation and pluripotency characterization of PASMC-derived iPSC lines. (A) Schematic overview of the reprogramming strategy used to convert pulmonary artery smooth muscle cells (PASMCs) into induced pluripotent stem cells (iPSCs). (B) Representative immunofluorescence staining for pluripotency markers OCT3/4, NANOG, and SSEA-4 in newly generated PVOD-derived iPSC lines (34L2, 34L3, 34L4). Scale bar: 100 um. (C) Quantitative PCR analysis of pluripotency-associated genes (SOX2 and NANOG) in PVOD-derived iPSC lines compared with a control iPSC line. (D) Spontaneous differentiation of PVOD-derived iPSCs followed by immunostaining for germ layer markers: alpha-fetoprotein (endoderm), desmin (mesoderm), and MAP2 (ectoderm). Scale bar: 20 um. (E) Flow cytometric analysis of germ layer differentiation following spontaneous differentiation. (human (iPSC lines derived from pulmonary artery smooth muscle cells of PVOD patients and healthy donors); FIGURE 1.)

Lian, Daniels, Wanner et al 2026, Frontiers in Cell and Developmental Biology · CC BY 4.0

Characterization of RhiPSC line W6 clone 5.07. (A) Amplicon sequencing of MAPT R406W with wild-type C allele indicated in the black box with a red arrow compared to reference genome (ref). (B) Phase contrast image of colonies grown on MEFs and (C) positive alkaline phosphatase staining; scale bar, 100 um. (D) RT-PCR detection of endogenous pluripotent mRNAs POU5F1, SOX2, NANOG, KLF4, LIN28A, and MYC. ACTB was used as a reference gene. (E) Amplification of EBNA and oriP in RhiPSCs. (F) Positive immunofluorescence staining for pluripotency proteins SOX2 (green), OCT4 (red), and NANOG (far red), colocalized with DAPI (blue). Scale bar, 100 um. (G) Flow cytometry-detected APC-A+ cells were 94.5% positive for SOX2, 99.2% positive for OCT4, and 95% positive for NANOG. (H) Short tandem repeat (STR) analysis confirmed RhiPSC line identity. Mycoplasma, Herpes B Virus (HBV), Simian Immunodeficiency Virus (SIV), Simian T-Lymphotropic Virus (STLV), and Simian Retrovirus (SRV) were not detected. (I) Normal 42,XX karyotype. (J) H&E staining of a teratoma containing endoderm, mesoderm, and ectoderm. Scale bar, 100 um. (rhesus macaque (iPSC line W6 clone 5.07 derived from rhesus macaque fibroblasts; MAPT R406W wild-type); FIGURE 4.)

Colwell, Maufort, Williams et al 2026, Frontiers in Cell and Developmental Biology · CC BY 4.0

Socs3 deficiency drives EpiSCs to naive ESCs conversion (A) Schematic diagram of the experimental workflow for reprogramming EpiSCs to rnESCs. (B) Primary Oct4-GFP+ colonies at day 14 in 2i/LIF. Scale bar, 100 um. (C) Quantification of rnESC colony number generated from wild-type (WT), Socs3 KO-1 and KO-2 EpiSCs after 14 days of reprogramming in 2i/LIF (n = 3, biological replicates). (D) Established Socs3 KO Oct4-GFP+ rnESC line displayed naive ESC morphology. Scale bar, 100 um. (E) Flow cytometry analysis of the Oct4-GFP+ cell proportion in Socs3 KO-1 and KO-2 rnESCs. E14Tg2a ESCs (WT) served as negative control. (F) qRT-PCR analysis of pluripotency marker genes (Oct4, Nanog) and ectoderm lineage marker genes (Pax6, Tuj1) of Socs3 KO rnESCs cultured in 2i/LIF and N2B27. (G) qRT-PCR analysis of endoderm lineage marker genes (Gata4, Gata6) and mesoderm lineage marker genes (Brachyury, Sox17) of Socs3 KO rnESCs cultured in M10 medium. (H) Immunofluorescence staining depicting the localization of PAX6 (ectoderm marker, red) in differentiated Socs3 KO cells. DAPI stains cell nuclei (blue). Scale bar, 100 um. (I) Immunofluorescence staining depicting the localization of alpha-SMA (mesoderm marker, green) and GATA4 (endoderm marker, red) in differentiated Socs3 KO rnESCs. DAPI stains cell nuclei (blue). Scale bar, 100 um. (FIGURE 3.)

Lu, Huang, Du et al 2026, Frontiers in Genetics · CC BY 4.0

Characterization of mESCs and rESCs derived and maintained in 6iL. a Top: representative phase-contrast images showing ESCs cultured in 6iL for 25 passages. Scale bars, 100 um. Bottom: AP staining of ESC colonies derived in 6iL. Scale bar, 50 um. b Representative immunofluorescence (IF) images of 6iL-derived ESCs (P15). Green indicates NANOG; red indicates OCT4; blue indicates Hoechst. Scale bars, 100 um. c UMAP projection showing transcriptional relationships among PSC states and embryonic reference populations. d Representative IF images of EB outgrowths derived from 6iL-mESCs stained for multilineage differentiation markers. Scale bars, 200 um. e Representative hematoxylin and eosin (H&E) staining of teratomas derived from 6iL-mESCs (P30), showing differentiated tissues representative of the three germ layers: endoderm, mesoderm, and ectoderm. Scale bars, 100 um. f Representative bright-field and fluorescence images of E9.5 chimera generated by injecting GFP-labeled 6iL-mESCs into blastocysts. Scale bars, 500 um. g Representative images of gonads isolated from E13.5 embryos. Arrowheads indicate gonads derived from chimeric embryos generated using GFP-labeled 6iL-mESCs. Scale bars, 500 um. h Representative IF images of rESCs cultured in 6iL, showing expression of pluripotency markers NANOG (red) and OCT4 (green). Hoechst (blue) marks nuclei. Scale bars, 50 um. i PCA plot showing transcriptomic relationships between mouse embryonic stages (E3.5-E6.5) and stem cell populations. (mouse (mESC) and rabbit (rESC) embryonic stem cells derived and maintained in 6iL/E4 culture conditions; Fig. 2.)

Wang, Ming, Yang et al 2026, Cell Research · CC BY 4.0

Characterization of induced pluripotent stem cell lines. (A) Karyotype analysis of hiPSC clones. (B) Cell surface expression of SSEA4 by flow cytometry (98%). (C) Morphology and immunofluorescence staining of pluripotency markers (NANOG and OCT4). (D) Immunofluorescence staining of germ layer markers (AFP as endoderm, SMA as mesoderm, and beta-III tubulin (TUB) as ectoderm markers). Scale bar size; black: 200 um; white: 100 um. (FIGURE 1.)

Vincze, Varga, Toth et al 2026, Pathology and Oncology Research · CC BY 4.0

Papers

Zhang Y, Ji J, Dong B, Zhu Z, Lv X, Chen P, et al. Chemical Screening Identifies Danofloxacin as a Self-Renewal Agonist of Embryonic Stem Cells through Alleviation of HDAC1-Mediated Deacetylation of Tert and Prdm10. Adv Sci (Weinh). 2026:e00624. PMID: 42801716.
Vincze K, Varga N, Tóth S, Kis AK, Béres R, Réthelyi JM, et al. Beyond animal models: using human pluripotent stem cell-derived stem and progenitor cells to predict cytotoxicity in normal tissues. Pathol Oncol Res. 2026;32:1612490. PMID: 42694232.
Lian L, Daniels N, Wanner N, Comhair S, Poplawski GHD, Asosingh K, et al. Induced pluripotent stem cells from sporadic pulmonary veno-occlusive disease exhibit altered endothelial commitment and stress-related transcriptional programs. Front Cell Dev Biol. 2026;14:1811995. PMID: 42609505.
Colwell JC, Maufort JP, Williams KM, Makulec AT, Fiorentino MV, Metzger JM, et al. Replicable generation and stable maintenance of rhesus macaque iPSCs for in vitro modeling of genetic frontotemporal dementia. Front Cell Dev Biol. 2026;14:1833030. PMID: 42597777.
Lu R, Huang S, Du M, Yang X, Liu J, Lin Z, et al. SOCS3 deficiency drives the primed to naive pluripotency transition by sustaining STAT3 activation. Front Genet. 2026;17:1857225. PMID: 42544337.
Wang D, Ming H, Yang D, Cui X, Freeman Z, Tsai LK, et al. A universal 6iL/E4 culture system for deriving and maintaining embryonic stem cells across mammalian species. Cell Res. 2026;36:611-628. PMID: 42443522.
Tong X, Chen X, Anicete A, Chan Y, Zhou X, Wang X, et al. Generation of Chimera-Competent Avian iPSCs Using Defined Transcription Factors. Cells. 2026;15:1092. PMID: 42346119.
Chen Q, Jiang X, Xue X, Zhou Y, Huang Y, Sun F, et al. Derivation and Pluripotency Validation of Six iPSC Lines From Amniotic Fluid Carrying Intermediate α-Thalassemia Genotypes (--3.7/αSEA and --4.2/αSEA). Stem Cells Int. 2026;2026:9951684. PMID: 42257012.
Beiner D, Zhu H, Bosholm CC, Wang HC, Criswell T, Atala A, et al. Derivation and characterization of retinal pigment epithelium from urine-derived iPSCs. FEBS Open Bio. 2026;16:1691-1707. PMID: 42030962.
Alsolami S, Chandrasekaran AP, Jin Y, Wang Y, Zhang L, Shakir IM, et al. A single small molecule-based human embryo model reveals V-ATPase requirement in mammalian blastocyst cavitation. Cell Res. 2026;36:475-498. PMID: 41936702.